As a result of the need to save energy and the increased consciousness of environmental problems, biotechnological production methods are on the advance. Fermenters are used for the biotechnological production of enzymes and other products using bacteria, yeast and cell cultures. For educational purposes a bubble bioreactor used in this experiment is a more convenient and economical alternative to commercial fermenters. To demonstrate how fermenters work, in this experiment molasse which is a waste product of sugar production is fermented in the so-called batch process.
ΠΠ΅ΡΠ΅ΡΠ΅Π½Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ² (Π²ΡΜΠΏΠΈΡΠΊΠ°) |
ΠΡΠΎΠ΄ΡΠΊΡ |
ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ |
Precision Balance, Sartorius ENTRIS822-1S, 820 g / 0,01 g
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49295-99
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1
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Immersion thermostat Alpha A, 230 V
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08493-93
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1
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Heating mantle f. roundbottom flask, 250ml
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49542-93
|
1
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Bubble bioreactor
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65999-00
|
1
|
Frame for complete experiments
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45500-00
|
1
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Power regulator
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32288-93
|
1
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Bath for thermostat, makrolon
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08487-02
|
1
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Liebig Condenser, with head, GL18/8
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35795-15
|
1
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Panel for complete experimental setups
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45510-00
|
2
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Apparatus carrier w. fix. magnet
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45525-00
|
1
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External circulation set for thermostat Alpha A
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08493-02
|
1
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Stutzer attachment GL25/12
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35791-15
|
1
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Apparatus holder, variable
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45526-00
|
1
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Aquarium pump, 150 l/h, 230 V AC
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64566-93
|
1
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Pycnometer, calibrated, 25 ml
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03023-00
|
1
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Clamping holder, turnable, 18-25 mm
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45521-00
|
1
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Flask,round,1-neck,250ml,GL25/13
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35812-15
|
1
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Round bottom flask, 100ml, GL 25/12
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35841-15
|
1
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Rear-cover for compl.-exp. panel
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45501-00
|
1
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Oleic acid pure 500 ml
|
31685-50
|
1
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Clamping holder,18-25mm
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45520-00
|
4
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Hook on fixing magnet
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02151-03
|
1
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pH test sticks 4.0-7.0,100 sticks
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30301-03
|
1
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Clamp for heating mantle
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49557-01
|
1
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Glass tube,right-angled, 10 pcs.
|
36701-52
|
1
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Tube Coupling, d = 8 mm
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47521-00
|
2
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Spring plugs, 50 off
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45530-00
|
1
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Pipettor
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36592-00
|
1
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Sulphuric acid, 10%, tech.gr., 1000 ml
|
31828-70
|
1
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Glass tubes,straight with tip, 10
|
36701-63
|
1
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Sodium hydroxide sol.,10%, 500ml
|
31630-50
|
1
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Magnesium sulphate 500 g
|
30136-50
|
1
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G-clamp
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02014-00
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2
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Dropping pipette with bulb, 10pcs
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47131-01
|
1
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Bottle,nar.mouth,1000ml,clear,p.s
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41104-01
|
1
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Ammonium sulphate 250 g
|
30027-25
|
1
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Fixing bands,universal,100 pcs.
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45535-00
|
1
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Cotton wool, white 200 g
|
31944-10
|
1
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Erlenmeyer flask,narrow n.,100 ml
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36118-00
|
1
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Erlenmeyer flask, DURAN, narrow neck, 250 ml
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36124-00
|
2
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Graduated cylinder 100 ml
|
36629-00
|
1
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Pinchcock, width 15 mm
|
43631-15
|
1
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Funnel, glass, top dia. 80 mm
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34459-00
|
1
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Spoon, special steel
|
33398-00
|
1
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Silicone tubing i.d. 7mm, 1 m
|
39296-00
|
1
|
Graduated pipette 10 ml
|
36600-00
|
1
|
Silicone tubing, ID 4 mm
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47529-00
|
1
|
Rubber tubing, i.d. 6 mm
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39282-00
|
6
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Tubing connector, ID 6-10mm
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47516-01
|
2
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Hose clip, diam. 8-16 mm, 1 pc.
|
40996-02
|
13
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Hose clip f.12-20 diameter tube
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40995-00
|
1
|